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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Targeting prokineticin system counteracts hypersensitivity, neuroinflammation, and tissue damage in a mouse model of bortezomib-induced peripheral neuropathy

Fig. 5

Effect of PC1 chronic treatment on macrophage activation and cytokine levels in DRG. a, b mRNA levels of CD68 and the percentage of CD68 positive area in DRG of CTR, BTZ 14 days (corresponding to c.d. 2.4 mg/kg), BTZ 28 days (corresponding to c.d. 4.8 mg/kg), and PC1 (BTZ + PC1) mice. Quantitative analysis of CD68 signal was computed as integrated optical density for arbitrary areas (6 sections per animal, 5 animals per group). One-way ANOVA was used for statistical evaluation, followed by Bonferroni’s test for multiple comparisons. ***p < 0.001 vs CTR; °°°p < 0.001 vs BTZ day 28. c Representative images of CD68 immunofluorescence signal in DRG sections while d immunofluorescence double staining images shows the colocalization (white arrowhead) of PK2 (green) with CD68 (activated macrophages, red) in CTR, BTZ 14 days, BTZ 28 days, and PC1 (BTZ + PC1) mice. eh the mRNA levels of TLR4 and of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6. i The anti-inflammatory cytokine IL-10. All the measurements were performed 14 days after the first BTZ administration, before starting PC1 treatment (CTR and BTZ groups), and at the end BTZ/BTZ + PC1 protocol (CTR, BTZ, BTZ + PC1 groups). (a, ei) Data represent mean ± SD of 6 mice/group. At day 14, statistical analysis was performed by means of t test while at day 28 by means of one-way ANOVA analysis of variance followed by Bonferroni’s post-test. *p < 0.05, **p < 0.01, ***p < 0.001 vs vehicle/CTR; °p < 0.05,°°p < 0.01, °°°p < 0.001 vs BTZ day 28

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