Fig. 3
From: Anti-CNTN1 IgG3 induces acute conduction block and motor deficits in a passive transfer rat model
Immunofluorescence staining of sciatic nerves of rats with Cy3-conjugated anti-human IgG (a, b), FITC-conjugated anti-human IgG3 (c) and IgG4 (d). Confocal fluorescence microscopy detects a small band of autoantibody binding at the paranodes of a patient animal (a), not in a control animal (b). Structural illumination microscopy reveals a tube-like arrangement of autoantibodies around the axon in the patient animal (c), not in the control animal (d). Subclass specific antibodies uncover IgG3 as the binding subclass in the acute patient (e) and IgG4 in the chronic patient (f). Bars in (a, b, e, f) = 10 μm, in (c) and (d) 5 μm, rectangles in (c) indicate the orientation