Fig. 2
From: Dysregulation of sonic hedgehog pathway and pericytes in the brain after lentiviral infection
Dysregulation of Shh with SIV infection double-label IF for Shh (green) and DAPI (blue) shows no significant difference between groups a. Triple-label IF of GLUT1 (red), Shh (green), and DAPI (blue) shows an increase in the MPI of Shh near the endothelium of both infection groups when compared to the control group (b). Likewise, IF imaging of Shh (green), GLUT1 (red), and DAPI (blue) in a SIVE lesion shows consistently high levels of Shh near the lesion-associated endothelium c. No significant difference was found in Shh levels between non-lesion-associated vessels and lesion-associated vessels in SIVE d. Twelve lesions were used from each SIVE animal (n = 6), and Shh MFI was compared between non-lesion and lesion-associated vessels in each animal. Lesions are circled with white dotted lines (c). Error bars denote SD