Fig. 4
From: Dysregulation of sonic hedgehog pathway and pericytes in the brain after lentiviral infection
Pericytes and netrin-1 dysregulated with SIV infection IF imaging of netrin-1 (green) and DAPI (blue) show an increase in netrin-1 MPI in SIVE animals (a). Double IF staining of PDGFRB (red) and GLUT1 (green) displays increased pericyte thickness in SIVE animals compared to uninfected (b). Double IF staining for PDGFRB (red), and DAPI (blue), shows that PDGFRB is eliminated in SIVE lesions (d). Likewise, staining for netrin-1 (green), and DAPI (blue), shows a similar elimination of netrin-1 in SIVE lesions c. Twelve lesions were used from each SIVE animal (n = 6) and PDGFRB area (c) or netrin-1 MPI (d) was compared between non-lesion and lesion-associated vessels in each animal. Lesions are circled within white dotted lines (c, d). Error bars denote SD