Fig. 5
From: Dysregulation of sonic hedgehog pathway and pericytes in the brain after lentiviral infection
Changes in pericyte thickness of SIVE brain are due to cellular changes, not pericyte number. Pericyte thickness graphed against the number of pericytic nuclei shows no significant trend between the number of pericytes and their thickness around the endothelium of SIVE infected animals (a). Total PDGFRB intensity shows no change in PDGFRB expression between study groups despite the significant difference in pericyte area (b). Triple-label IF for PDGFRB (red), GLUT1 (green), and DAPI (blue) of an expanded pericyte (c) and a non-expanded pericyte (d) show the cellular differences between two pericytes in the same SIVE animal (10A067). Using double-label IF for PDGFRB (red) and fibrinogen (green), we were able to determine that 80% of vessels surrounded by enlarged pericytes showed signs of fibrinogen extravasation (e, f). Error bar denotes SD. Ten vessels were randomly chosen from each SIVE animal (n = 6); each point on the graph a denotes a single vessel. No significant difference was found in a or b. A paired t test was used to compare between samples in f