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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Dysregulation of sonic hedgehog pathway and pericytes in the brain after lentiviral infection

Fig. 7

Findings in rhesus macaques confirmed in human tissue triple IF for Shh (green), GLUT1 (red), and DAPI (blue) show increased IR of Shh in association with the vessels of HIVE patients when compared to UI a and remained high in lesion-associated vessels (c). Likewise, double IF for GLUT1 (green) and PDGFRB (red) shows an increase in pericyte thickness in HIVE patients (b). Despite this increase, however, triple IF for HIVp24 (green), PDGFRB (red), and DAPI (blue) demonstrates a marked lack of PDGFRB+ pericytes in lesions (d). Further investigation of the same IF stain shows HIV Gag p24 localized to hypertrophied pericytes. The presence of HIVp24 within human brain pericytes was confirmed via a confocal microscopy Z-stack using 0.25 μm increments and an orthogonal view representation e. Lesions are circled with white dotted lines (d). A paired t test was used to determine significance between groups in a and b

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