Fig. 3

Overexpression of 1810034E14Rik downregulated inflammatory factors in microglial cells. a The primary microglial cells were transferred with Lv-1810034E14Rik or negative control 24 h before OGD 4 h/R 24 h induce. The expression of lncRNA-1810034E14Rik was detected by RT-qPCR. The mRNA levels of cytokines IL-1b (b), TNF-α c, IL-6 (d), IL-10 (e), and IL-4 (f) in primary microglial cells were determined by RT-qPCR. Cytokines such as TNF-α (g), IL-1b (h), IL-6 (i), IL-4 (j), and IL10 (k) secreted by microglial cells were tested by ELISA. The data represents mean ± SEM. n = 15, *P < 0.05, **P < 0.01, and ***P < 0.001 versus the control group; ^##P < 0.01, ^###P < 0.001 versus the OGD group + Lv-control group; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001 versus the OGD + siControl group