Fig. 4

1810034E14Rik ameliorated microglial cells induced neuron injury. a Supernatant culture of microglial cells with different treatment was collected and added to 5-day-old neurons at 1:4. Neuron death was measured by LDH released assay. b Cell counting Kit-8 assay of neurons treated with the culture of microglial cells. c Representative double-staining immunofluorescence of NeuN and cleaved-caspase3 in neurons. d Calcein-AM/PI co-staining immunofluorescence of Neurons. The data represents mean ± SEM. n = 15, *P < 0.05, **P < 0.01, and ***P < 0.001 versus the control group; ^##P < 0.01, ^###P < 0.001 versus the OGD group + Lv-control group; ^P < 0.05, ^^P < 0.01, ^^^P < 0.001 versus the OGD + siControl group