Fig. 1
Determination of MIF protein levels and PGE2 production following spinal cord injury. a Western blot analysis of MIF, COX1, and COX2 protein levels following spinal cord contusion at 0 day, 1 day, 4 days, and 7 days, respectively. b–e Determination of COX1 (b), COX2 (c), mPGES-1, mPGES-2, and cPGES (d, e) protein levels for the injured cord at different time points with or without injection of 8 μl 4-IPP (100 mM) at the lesion sites. Quantities were normalized to endogenous β-actin. f Production of PGE2 was assayed by ELISA accordingly. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05; #P < 0.05)