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Fig. 10 | Journal of Neuroinflammation

Fig. 10

From: Macrophage migration inhibitory factor facilitates prostaglandin E2 production of astrocytes to tune inflammatory milieu following spinal cord injury

Fig. 10

Effects of COX2 inhibitor NS398 on the production of inflammatory cytokines from macrophages in vitro or at spinal cord lesioned site. af ELISA assay determined production of TNF-α (a, b), IL-1β (c, d), and IL-6 (e, f) following macrophages cultured with astrocytes-conditioned medium in the presence of 1 μg/ml LPS for 24 h. The astrocytes were cultured in the presence of 1 μg/ml recombinant MIF with or without 30 μM NS398 for 24 h. g Determination of PGE2 production at lesion sites of spinal cord following injection of 10 μl 80 mM NS398 at 0 day, 1 day, 4 days, and 7 days, respectively. h, i ELISA assay determined production of TNF-α (h) and IL-1β (i) at lesion sites following injection of 10 μl 80 mM NS398 at 0 day, 1 day, 4 days, and 7 days, respectively. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05; #P < 0.05)

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