Fig. 3
Determination of PGE2 synthesis-related protein levels in response to recombinant MIF stimulation. a Showing isolated astrocytes stained with GFAP and Hoechst 33342 with purity more than 95%. b Western blot analysis of COX1, COX2, mPGES-1, mPGES-2, and cPGES following astrocytes treatment with 0–2.5 μg/ml recombinant MIF for 24 h. c, d Quantification data as shown in (b). Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05). Scale bars, 50 μm in (a)