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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Macrophage migration inhibitory factor facilitates prostaglandin E2 production of astrocytes to tune inflammatory milieu following spinal cord injury

Fig. 5

Effects of COX2 inhibitor NS398 on the MIF-induced expression of PGE2 synthesis-related protein. a Western blot analysis of COX1, COX2, mPGES-1, mPGES-2, and cPGES following astrocytes treatment with 1 μg/ml recombinant MIF for 24 h in the presence of 30 μM NS398. b, c Quantification data as shown in (a). d, e Cell supernatants (d) and lysates (e) were tested by ELISA for the production of PGE2, following astrocytes treatment with 1 μg/ml recombinant MIF for 24 h in the presence of 30 μM NS398. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05; #P < 0.05)

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