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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Macrophage migration inhibitory factor facilitates prostaglandin E2 production of astrocytes to tune inflammatory milieu following spinal cord injury

Fig. 6

Effects of CD74 knockdown on the MIF-induced expression of PGE2 synthesis-related enzymes. a Western blot analysis of CD74, COX1, COX2, mPGES-1, mPGES-2, and cPGES following siRNA2 knockdown of CD74 receptor for 48 h, and then treated with 1 μg/ml recombinant MIF at 6 h and 12 h, respectively. A siRNA (scramble) with the same nucleotide composition as siRNA2 but which lacks sequence homology to the CD74 was also designed as negative control. b, c Quantification data as shown in (a). d, e Cell supernatants (d) and lysates (e) were tested by ELISA for the production of PGE2, following knockdown of CD74 receptor for 48 h, and then treated with 1 μg/ml recombinant MIF at 6 h and 12 h, respectively. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05)

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