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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: Macrophage migration inhibitory factor facilitates prostaglandin E2 production of astrocytes to tune inflammatory milieu following spinal cord injury

Fig. 7

Determination of intracellular signaling associated with regulation of PGE2 production. a Western blot analysis of COX1, COX2, mPGES-1, mPGES-2, and cPGES following astrocytes treatment with 1 μg/ml recombinant MIF for 24 h in the presence of 10 μM ERK (PD98059), 10 μM P38 (SB203580), or 10 μM JNK (SP600125) inhibitor. bd Quantification data as shown in (a). e, f Cell supernatants (e) and lysates (f) were tested by ELISA for the production of PGE2, following astrocytes treatment with 1 μg/ml recombinant MIF for 24 h in the presence of 10 μM ERK (PD98059), 10 μM P38 (SB203580), or 10 μM JNK (SP600125) inhibitor. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05; #P < 0.05)

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