Fig. 7
Determination of intracellular signaling associated with regulation of PGE2 production. a Western blot analysis of COX1, COX2, mPGES-1, mPGES-2, and cPGES following astrocytes treatment with 1 μg/ml recombinant MIF for 24 h in the presence of 10 μM ERK (PD98059), 10 μM P38 (SB203580), or 10 μM JNK (SP600125) inhibitor. b–d Quantification data as shown in (a). e, f Cell supernatants (e) and lysates (f) were tested by ELISA for the production of PGE2, following astrocytes treatment with 1 μg/ml recombinant MIF for 24 h in the presence of 10 μM ERK (PD98059), 10 μM P38 (SB203580), or 10 μM JNK (SP600125) inhibitor. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05; #P < 0.05)