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Fig. 9 | Journal of Neuroinflammation

Fig. 9

From: Macrophage migration inhibitory factor facilitates prostaglandin E2 production of astrocytes to tune inflammatory milieu following spinal cord injury

Fig. 9

Blocking EP2 receptor attenuated PGE2-mediated function on production of inflammatory cytokines. a Expression analysis of PGE2 receptors by RT-PCR following macrophage treatment with 1 μg/ml LPS for 24 h; ELISA assay was used to determine TNF-α (b), IL-1β (c), or IL-6 (d) production in the supernatants and lysates of macrophage RAW 264.7 following treatment with 1 μg/ml LPS and 2.5 μM PGE2 in the presence or absence of 10 μM EP2 inhibitor PF-04418948. Experiments were performed in triplicates. Error bars represent the standard deviation (*P < 0.05; #P < 0.05)

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