Fig. 1From: Icariin attenuates neuroinflammation and exerts dopamine neuroprotection via an Nrf2-dependent mannerICA ameliorated 6-OHDA-caused DA neuronal damage. Mice were intragastrically given ICA (60 mg/kg) for 10 consecutive days and a single intranigral injection of 6-OHDA (4 μg) in the left side of SN beginning 3 days after ICA treatment. After the last treatment of ICA, rotarod test (a) and open-field test (b) were performed. TH-positive neurons were measured by immunofluorescence staining (c). TH protein expression in midbrain was tested by western blot assay (d). The levels of DA, DOPAC and HVA in striatal tissues were detected by UHPLC. Meanwhile, the DA metabolite ratio [(DOPAC + HVA)/DA × 100] was computed (e). Results were mean ± SEM from 5 to 6 mice. *p < 0.05 compared with the control group, #p < 0.05 compared with 6-OHDA-treated group. Scale bar = 200 μmBack to article page