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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Ultrastructural evidence of microglial heterogeneity in Alzheimer’s disease amyloid pathology

Fig. 4

Distinct changes in microglial phagolysosomal system of APPSwe-PS1Δe9 animals. ac Example of IBA1-positive microglial cell bodies containing empty inclusions, non-empty inclusions (pseudocolored in green), or fibrillar materials in wild-type (WT; a), APP-D (b), or APP-P (c) subregions of ventral hippocampus CA1. d Quantification of fibrillar materials phagocytosis by microglial cell bodies in APPSwe-PS1Δe9 mice. e Quantification of cell bodies containing empty phagocytic inclusions in APPSwe-PS1Δe9 mice. f Only microglial cell bodies in APP-P regions contained fibrillar materials. g Cell bodies in APP-P regions contained increased numbers of non-empty phagocytic inclusions. hj IBA1-positive microglial processes containing various phagocytic inclusions in APP-P and APP-D subregions. k Quantification of microglial process inclusions in WT and APPSwe-PS1Δe9 mice. l Quantification of microglial processes containing empty inclusions in APPSwe-PS1Δe9 mice. m Only microglial processes in APP-P regions contained fibrillar materials. n Quantification of lysosome numbers in APP-H, APP-D, and APP-P subregions associated microglial processes. d dendrite, Ep empty phagocytic inclusion, dn dystrophic neurite, fm fibrillar material, Ex extracellular space pocket containing debris, m microglia, mt mitochondrion, s dendritic spine, t axon terminal. Error bars represent mean ± SEM. WT levels are shown on the subregion graphs by a gray horizontal line. n = 50 processes per condition for APP-H, APP-D, and APP-P and 70 processes per animal for WT animals, n = 7–15 cells per condition for all conditions, and data was collected from N = 3–4 animals per condition. * denotes difference from WT, ~ denotes difference from APP-H, # denotes difference from APP-P *,~p < 0.05; **,~~p < 0.01; ***,~~~,###p < 0.001

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