Fig. 5
From: Ultrastructural evidence of microglial heterogeneity in Alzheimer’s disease amyloid pathology
Microglia in APPSwe-PS1Δe9 animals display increased signs of cellular stress. a Example of IBA1-positive cell body imaged in the ventral hippocampus CA1 of the APP-P subregion that displays ER dilation, a lipidic inclusion and lipofuscin granules. b Example of microglial process in the APP-P subregion showing ER dilation. c IBA1-positive microglial cell body contacting extracellular space pockets with debris and containing lipidic inclusions and lipofuscin granules. d Microglial cell process containing a lysosome and a lipidic inclusion. d dendrite, er endoplasmic reticulum, Ex extracellular space pocket containing debris, Lg lipofuscin granule, Lip lipidic inclusion, Lys lysosome, m microglia, ma myelinated axon, mt mitochondrion, s dendritic spine, t axon terminal. e Quantification of dilated ER in microglial cell bodies from APP-H, APP-D, and APP-P subregions of APPSwe-PS1Δe9 mice. f Microglial processes in APPSwe-PS1Δe9 animals are more likely to contain lipidic inclusions than those in WT animals. g Quantification of lipid body inclusions in microglial processes from APP-H, APP-D, and APP-P subregions. n = 50 processes per condition for APP-H, APP-D, and APP-P and 70 processes per animal for WT animals, n = 7–15 cells per condition for all conditions, and data was collected from N = 3–4 animals per condition. Error bars represent mean ± SEM. WT levels are shown on the subregion graphs by a gray horizontal line. #p < 0.05; ***,###p < 0.001. * denotes difference from WT animal, # denotes difference from APP-P region