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Table 3 Ultrastructural analysis of microglial processes in wild-type (WT) littermate controls versus APPSwe-PS1Δe9 mice

From: Ultrastructural evidence of microglial heterogeneity in Alzheimer’s disease amyloid pathology

  WT APP
Mean ± SEM Mean ± SEM
Area (μm2) 0.4111 ± 0.021 0.3957 ± 0.020
Perimeter (μm) 3.476 ± 0.13 3.409 ± 0.13
Circularity (a.u.) 0.465 ± 0.1 0.499 ± 0.01*
Roundness (a.u.) 0.483 ± 0.009 0.508 ± 0.009
Solidity (a.u.) 0.761 ± 0.008 0.780 ± 0.007*
Lipidic inclusion (n) 0.139 ± 0.025 0.336 ± 0.035***
Fibrillar materials inclusion (n) 0 0.0133 ± 0.007
Phagocytic process (%) 0.3179 ± 0.024 0.2289 ± 0.02
Total inclusion (n) 0.4923 ± 0.043 0.3578 ± 0.037
Non-empty inclusion (n) 0.210 ± 0.026 0.189 ± 0.027
Empty inclusion (n) 0.292 ± 0.032 0.182 ± 0.024
ER dilation (n) 0.021 ± 0.008 0.04 ± 0.009
Extracellular debris (n) 0.380 ± 0.038 0.249 ± 0.027*
Total lysosome (n) 0.0153 ± 0.0062 0.0422 ± 0.015
Primary lysosome (n) 0.005 ± 0.003 0.009 ± 0.007
Secondary lysosome (n) 0.01 ± 0.005 0.03 ± 0.009
Tertiary lysosome (n) 0 0.016 ± 0.008
Encircled Aβ (n) 0 0.016 ± 0.009
Encircled synaptic element (n) 0.085 ± 0.02 0.20 ± 0.03**
Encircled dendritic spine (n) 0.06 ± 0.016 0.10 ± 0.018*
Encircled axonal terminal (n) 0.02 ± 0.008 0.08 ± 0.01***
Encircled degraded structure (n) 0.015 ± 0.007 0.029 ± 0.009
  1. a.u. arbitrary unit, n number per microglial process