Fig. 10

A discussion of the potential targets of baicalin. a, b BV2 cells were pretreated with LY 292002 (10 μM) and compound C (20 μM) and co-incubated with baicalin (100 μM) for 1 h and then stimulated with LPS (1 μg/ml) for 24 h. Western blot was performed to detect the protein levels of p-AKT, TLR4 (a, n = 4), p-AMPK, and TLR4 (b, n = 4), and a statistical analysis was conducted. Data were normalized to the blank group and presented as means ± SEM. *p < 0.05, **p < 0.01 vs. blank; ^#p < 0.05, ^##p < 0.01 vs. LPS; ^&p < 0.05, ^&&p < 0.01 vs. LPS+BA60. c Western blot was performed to measure the levels of p-AKT and p-GSK3β in the hippocampus of CUMS mice after treatment with LY294002 and baicalin (n = 4). Data are presented as means ± SEM. *p < 0.05, **p < 0.01 vs. control; ^#p < 0.05, ^##p < 0.01 vs. CUMS; ^&p < 0.05, ^&&p < 0.01 vs. CUMS+BA60