Fig. 10

Effect of IL-10 on the lesion area and neuronal degeneration in wild-type mice treated with glutamate. Histological sections of wild-type (WT) mice were obtained after 24 h of intracerebral administration of NaCl 0.9% (S.S.), only glutamate 1 M (GLUT) or glutamate + 0.4 ng/mL of interleukin-10 (GLUT+IL-10) as detailed in the “Methods” section. a Representative micrographs of coronal striatal sections showing lesions stained with cresyl violet and damaged cells positive for Fluoro-Jade B. The dotted line delimits the area of lesion of mice treated with glutamate. The scale bars represent 200 μm. b Quantification of the lesion volume 24 h after saline or glutamate injection is expressed in cubic millimeters. c Total number of FJB-positive cells counted in three slices per mice. Data are expressed as mean ± SEM of six independent experiments. *p < 0.05 vs saline control (S.S.); ^ϕp < 0.05 vs the corresponding mouse treated only with glutamate (GLUT)