Fig. 9

Caspase-3 activity in wild-type mice treated with IL-4 after glutamate administration. Active caspase-3 was measured in striatal homogenates of wild-type (WT) mice treated with NaCl 0.9% (S.S.), only glutamate (− IL-4) or glutamate + 0.7 ng/mL of interleukin-4 (+IL4) after 1, 3, 6, 12, and 24 h. Fluorescence produced by the cleavage of the specific substrate for caspase-3 was quantified as relative units of fluorescence (FRU). Data are expressed as fold change of fluorescence relative to saline without IL-4. Values are means ± SEM of three independent experiments. *p < 0.05 vs the corresponding saline control; ^ϕp < 0.05 vs the corresponding time of mice treated with glutamate alone