Fig. 10

Axon tracing in the corticospinal tracts. Twenty-eight days post-SCI, rats were injected with BDA in the motor cortex and harvested 21 days after injection. Transverse sections rostral to the T10 level had an average of 739 ± 58 labeled axons in the dorsal corticospinal tract (a, b) and uninjured rats had an average of 97 ± 56 in the region caudal to the T10 level (c), which was significantly more than all other groups (P < 0.0001). Virtually no axons were labeled in the dorsal corticospinal tract caudal to the injury site in Controls (d) or any of the treatment groups and there was no significant difference between any of the groups that underwent an SCI. Red represents BDA labeled axons; blue represents DAPI; n = 3 per group; scale bars = 500 μm (a), 100 μm (b–d)