Fig. 12

Analysis of IL-10 effect on lesion size and spinal atrophy. Sagittal sections of spinal cord containing the injury site were sectioned 10 μm thick and GFAP positive astrocytes were labeled in Controls (a), Systemic IL-10 (b), Local IL-10 (c), MCMs (d), and MCMs+IL-10 (e). The spinal cord thickness was measured 5 mm rostral to the epicenter, at the epicenter, and 5 mm caudal to the epicenter and the percent in reduced thickness at the injury site was calculated (f). The MCMs+IL-10 was the only group with significantly less spinal atrophy than Controls. The infarct was measured as the area of tissue outlined by reactive astrocytes (g). Both the Systemic IL-10 and MCMs+IL-10 had a significantly smaller infarct area when compared to the Controls. *P < 0.05 (Dunnett’s Test); error bars represent ± SEM; n = 4; scale bar = 1 mm