Fig. 3From: Experimental necrotizing enterocolitis induces neuroinflammation in the neonatal brainExperimental NEC affects brain cell populations in a region-specific manner. a–c Immunohistochemistry images of mature neurons in breastfed control and NEC pups (left) using the antibody NeuN (Table 1) and their quantification (right). a Compared to breastfed control, the number of mature neurons was reduced in the hippocampus (a) and in the basal ganglia/thalamus in NEC pups (b). c Differences in the number of neurons between NEC and breastfed control pups were not significant in the cerebral cortex. d–f Immunohistochemistry images of oligodendrocytes in breastfed control and NEC pups using the antibody Olig2 (Table 1) (left) and their quantification (right). Compared to breastfed control, the number of oligodendrocytes was reduced in the hippocampus (d), in the basal ganglia/thalamus (e), and in the cerebral cortex of NEC pups (f). g–i Immunohistochemistry images (left) of neural progenitor cells in breastfed control and NEC pups using the antibody Sox2 (Table 1) and their quantification (right). Compared to breastfed control, the number of neural progenitor cells was reduced in the hippocampus (g), in the basal ganglia/thalamus (h), and in the cerebral cortex of NEC pups (i). Scale bar = 100 μmBack to article page