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Table 1 Antibodies used for immunohistochemistry and Western blotting

From: Experimental necrotizing enterocolitis induces neuroinflammation in the neonatal brain

Primary antibody Description Species Dilution Company Catalog number
NeuN Mature neurons Mouse 1:100 EMD Millipore Sigma MAB377
Olig2 Oligodendrocytes/progenitors Rabbit 1:200 abcam ab109186
Sox2 Neural progenitor cells Rabbit 1:400 abcam ab97959
Iba1 Microglia/macrophages Rabbit 1:4000 abcam ab178847
GFAP Astrocytes/ependymal cells Rabbit 1:200 Dako 20047046
IL-6 Interleukin-6 Rabbit 1:1000 abcam ab7737
TNFα Tumor necrosis factor-alpha Rabbit 1:1000 Cell Signaling Technology 11948
GAPDH Glyceraldehyde 3-phosphate dehydrogenase Rat 1:1000 Santa Cruz Biotechnology sc-32233
β-actin Beta-actin Rabbit 1:1000 Cell Signaling Technology 4970
CC3 Cleaved caspase-3 Rabbit 1:500 Cell Signaling Technology 9930
BiP immunoglobulin-binding protein Rabbit 1:1000 Cell Signaling Technology 3177
CHOP CCAAT-enhancer-binding protein homologous protein Rabbit 1:1000 Cell Signaling Technology 2895