Table 1 Antibodies used for immunohistochemistry and Western blotting
From: Experimental necrotizing enterocolitis induces neuroinflammation in the neonatal brain
Primary antibody | Description | Species | Dilution | Company | Catalog number |
|---|---|---|---|---|---|
NeuN | Mature neurons | Mouse | 1:100 | EMD Millipore Sigma | MAB377 |
Olig2 | Oligodendrocytes/progenitors | Rabbit | 1:200 | abcam | ab109186 |
Sox2 | Neural progenitor cells | Rabbit | 1:400 | abcam | ab97959 |
Iba1 | Microglia/macrophages | Rabbit | 1:4000 | abcam | ab178847 |
GFAP | Astrocytes/ependymal cells | Rabbit | 1:200 | Dako | 20047046 |
IL-6 | Interleukin-6 | Rabbit | 1:1000 | abcam | ab7737 |
TNFα | Tumor necrosis factor-alpha | Rabbit | 1:1000 | Cell Signaling Technology | 11948 |
GAPDH | Glyceraldehyde 3-phosphate dehydrogenase | Rat | 1:1000 | Santa Cruz Biotechnology | sc-32233 |
β-actin | Beta-actin | Rabbit | 1:1000 | Cell Signaling Technology | 4970 |
CC3 | Cleaved caspase-3 | Rabbit | 1:500 | Cell Signaling Technology | 9930 |
BiP | immunoglobulin-binding protein | Rabbit | 1:1000 | Cell Signaling Technology | 3177 |
CHOP | CCAAT-enhancer-binding protein homologous protein | Rabbit | 1:1000 | Cell Signaling Technology | 2895 |