Fig. 4

Deficiency of TREK-1 increases the MMP-9 protein expression but have no change on TJPs expression on days 1 and 3 after ICH compared with WT ICH group. a The immunofluorescent staining of the TJPs (ZO-1, occludin, claudin-5) on day 3 after ICH. Scale bar = 20 μm. b Representative western blots image of ZO-1, occludin, claudin-5, and MMP-9. c–f Quantifications of ZO-1, occludin, claudin-5, and MMP-9 protein levels in sham, ICH 1-day, and 3-day groups after ICH. All interest protein expression were normalized to β-actin. Values are expressed as mean ± SEM (n = 5), and data were evaluated by one-way ANOVA with Tukey post hoc test. ^#p < 0.05, WT sham group versus KO ICH group, *p < 0.05, WT ICH group versus KO ICH group