Fig. 5

Deletion of TREK-1 aggravates microglia cell activation, neutrophil infiltration, and promotes the secretion of pro-inflammatory cytokine IL-1β and TNF-α on 1 day and 3 days after ICH. Immunofluorescent staining of MPO (a) and Iba-1 (b) with DAPI in the perihematoma region to detect activated microglia and infiltrated neutrophils on days 1 and 3 after ICH. Scale bar = 20 μm. c–d Statistical analysis of the percentage of activated microglia and infiltrated neutrophils. e–f ELISA result of TNF-α and IL-1β in the perihematoma zone on days 1 and 3 after ICH. Values are expressed as mean ± SEM (n = 5–7), and data were evaluated by Student’s independent sample t test and one-way ANOVA with Tukey post hoc test. *p < 0.05, **p < 0.01 WT ICH group versus KO ICH group, ^#p < 0.05, WT SHAM group versus KO ICH group