Fig. 1

Schematic summary of the experimental procedure. The following steps were undertaken in both studies: Preterm delivery of rabbit pups via cesarean section on E29 (0 h); induction of IVH by i.p. administration of 50% (v/v) sterile glycerol (6.5 g/kg) at approx. 3 h of age; detection of IVH by high-frequency ultrasound. Feeding and verification of IVH, by high-frequency ultrasound, were performed throughout the study. For details about each step, see the “Materials and methods” section. a rA1M distribution. Randomization into study groups was done at approx. 18 h of age; i.c.v. administration of rA1M/Vehicle solution at approx. 22 h of age; termination and perfusion fixation of pups at 72 h of age. b hA1M functional protection. Randomization into study groups was done at approx. 6 h of age; i.c.v. administration of hA1M/Vehicle solution at approx. 8 h of age; termination of pups at 24 and 72 h of age