Fig. 6
hA1M reduces inflammatory response, cellular activation, oxidative stress and matrix degradation proteins. Rabbit pups with confirmed IVH, i.c.v. injected with hA1M (IVH + hA1M, dark grey bars; n = 9) or Vehicle (IVH + Vehicle, grey bars; n = 8) or Sham Control (white bars; n = 17; no bleeding as confirmed with high-frequency ultrasound) were euthanized at 72 h of age and the brains were removed from the skulls and a piece of periventricular tissue was carefully removed from the lateral ventricles, snap frozen, and the mRNA expressions of MCP-1 (a), IL-1β (b), IL-8 (c), TNFα (d), IL-6 (e), TLR-4 (f), IL1R1 (g), MMP-9 (h) and HO-1 (i) were subsequently analyzed with real-time PCR, as described in the “Materials and methods” section. mRNA expressions for the respective genes were normalized against those of GAPDH and are given as fold change. The fold change values were calculated by normalizing against samples from control animal (Sham Control). Results are presented as box plots displaying medians and 25th and 75th percentiles. Differences between IVH + hA1M vs. IVH + Vehicle and IVH + Vehicle vs. Sham Control at 72 h were analyzed using ANOVA post hoc Bonferroni. *P < 0.05, **< 0.01, ***< 0.001