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Table 3 Antibodies for immunohistochemistry and fluorescence staining

From: TRPV1 mediates astrocyte activation and interleukin-1β release induced by hypoxic ischemia (HI)

Antibody

Host

Company

Cat. no.

Dilution

Applied

Duration

GFAP

Mouse

Cell signaling

#3670

1:300

ICC

Overnight 4 °C

Iba1

Goat

NOVUS

NB100-1028

1:200

ICC

Overnight 4 °C

NRLP3

Rabbit

NOVUS

NBP2-12446

1:50

ICC

Overnight 4 °C

ASC

Mouse

Santa Cruz Bio.

SC-271054

1:50

ICC

Overnight 4 °C

Caspase-1

Mouse

Santa Cruz Bio.

SC-398715

1:50

ICC

Overnight 4 °C

Il-1β

Rabbit

Bio-Swamp

PAB30679

1:50

ICC

Overnight 4 °C

Anti-rabbit IgG-Cy3

Goat

Protein tech

SA00009-2

1:50

ICC

1 h 37 °C

Anti-mouse IgG-488

Goat

EarthOx

E032210-01

1:200

ICC

1 h 37 °C

DAPI

 

Beyotime

C1002

1;2000

ICC,IHC

1 min RT