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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Hydrogen-rich saline promotes microglia M2 polarization and complement-mediated synapse loss to restore behavioral deficits following hypoxia-ischemic in neonatal mice via AMPK activation

Fig. 1

Effects of HS on AMPK activation. a BV-2 cells treated with different concentration of HS for 2 h. b BV-2 cells treated with 1 μM HS for different times. c BV-2 cells treated with 1 μM HS with/without 500 ng/mL LPS for 2 or 4 h. d BV-2 cells treated with 1 μM HS with/without 500 ng/mL LPS or 1 μM Compound C (CC) for 2 h. Levels of p-AMPK, AMPK and β-actin were determined with use of Western blot. e Representative immunoblots of protein levels for phosphorylated AMPK (p-AMPK), AMPK and β-actin in ipsilateral cortex at 3 days following HI insult. N = 4/group. Values represent the mean ± SD, *p < 0.05, **p < 0.01 according to ANOVA

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Journal of Neuroinflammation

ISSN: 1742-2094