Fig. 4

Effect of HS on NF-κB activation in lesioned cortex and BV-2 cells. a Representative immunoblots are shown for phosphorylated NF-κB (p-NF-κB), total NF-κB, phosphorylated-IκBα (p-IκBα), and total IκBα within ipsilateral cortex at 3 days following HI insult as determined with western blotting analysis. Quantification of the relative levels of p-NF-κB/NF-κB, p-IκBα/ IκBα within each group. N = 4/group. b BV-2 cells treated with 1 μM HS and with/without 500 ng/mL LPS for 2 h. The level of p-NF-κB/NF-κB, p-IκBα/ IκBα were measured by Western blot. N = 4/group. Values were normalized to β-actin. Values represent the mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001 according to ANOVA