Fig. 4

Punctate OPN-immunoreactive profiles and two glial cells 14 and 28 days after 3-NP injection. a–d Triple labeling with OPN, GFAP, and Iba1 at day 14 post-lesion. Notably, OPN puncta appear to overlap with Iba1 expression in the lesion core but are also detectable within the astroglial scar, predominantly along its border immediately abutting the lesion core. Note that the astroglial scar is demarcated by the presence of hypertrophied and elongated astroglial processes, forming a network. e–h Triple labeling with OPN, GFAP, and Iba1 at day 28 post-lesion showing that OPN puncta are distributed throughout the astroglial scar, and are particularly abundant in areas forming a prominent, dense, compact scar. Cell nuclei appear blue after DAPI staining. Scale bars = 100 μm for a–h