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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Osteopontin and its spatiotemporal relationship with glial cells in the striatum of rats treated with mitochondrial toxin 3-nitropropionic acid: possible involvement in phagocytosis

Fig. 6

Representative images showing the intracytoplasmic OPN puncta in astrocytes and microglia/macrophages within the astroglial scar. af Three-dimensional views of triple labeling for OPN, Iba1, and GFAP from confocal z-stack images at 28 days post-lesion. a, d shows the relationship of OPN puncta with reactive astrocytes (a) or activated microglia/macrophages (d). b, e Images subtracting the OPN puncta outside glial cells from a and d, respectively. c, f Images showing the intracytoplasmic OPN puncta in astrocytes (c) and activated microglia/macrophages (f). g, i Orthogonal views showing the presence of OPN puncta within reactive astrocytes (magenta arrowheads in b, c, g, and h) or activated microglia/macrophages (yellow arrowheads in e, f, i, and j; white arrows in e, f, and h). h, j The three-dimensional rendering of intracytoplasmic OPN puncta in astrocytes (magenta arrowhead) and activated microglia/macrophages (yellow and white arrowheads). Cell nuclei appear blue after DAPI staining. Scale bars = 10 μm for af and g, i; 5 μm for h and j

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