Fig. 6

Synaptophysin protein levels undergo recovery after LPS removal. A diagrammatic representation of the LPS recovery experiment (a). OHSCs are aged for 2 weeks in vitro before undergoing 1 week of 200 ng/ml LPS. At 3 weeks in vitro, some slices are harvested to represent a 0 week after LPS removal timepoint. All other cultures undergo a 100% medium exchange to LPS-free medium. Slices are then harvested at either 1 week or 2 weeks after LPS removal and synaptophysin protein levels assessed by western blot (b). Slices harvested with no recovery after LPS removal showed a reduction in synaptophysin levels when compared to untreated samples (*p = 0.014). At 1 week (p = 0.30) or 2 weeks (p = 0.13) after LPS removal, there is no significance between LPS-exposed and untreated OHSCs (c). Analysis was conducted using a two-way ANOVA (n = 5–6 per timepoint and treatment group). Error bars = mean ± SEM