Fig. 2

APN suppressed AβO-induced proinflammatory cytokines release in BV2 cells. a, b BV2 cells were treated with different concentrations of AβO (0, 2.5, 5, 10, and 20 μM) for 24 h, and then ELISA assays of TNFα and IL-1β were conducted. c, d Cells were pretreated with different concentrations of APN (0, 2.5, 5, and 10 μg/ml) for 2 h, followed by exposure of AβO (10 μM) for another 24 h, and then ELISA assays of TNFα and IL-1β were conducted. Data were presented as the mean ± SEM for at least three independent experiments, and each performed in duplicates (n = 3). One-way ANOVA with Tukey’s multiple comparison test revealed a difference between groups. *p < 0.05, **p < 0.01, ***p < 0.001