Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Deletion of p38α MAPK in microglia blunts trauma-induced inflammatory responses in mice

Fig. 3

TBI-mediated recruitment of inflammatory monocytes is significantly reduced in mice lacking microglial p38α. Using flow cytometric analyses, we quantified the proportional change in cellular infiltrate into the ipsilateral hemisphere of mice 1 day following injury. Inflammatory monocytes were defined by a sequential gating strategy to enumerate CD11+Ly6C+ cell population in all four groups (WT and p38α KO, sham, and TBI). There were little-to-none inflammatory monocytes in the sham brains of either WT or KO mice. However, there was a robust increase observed in this cell population 1 day following TBI in WT mice, and this pathophysiological response was significantly blunted by ~ 50% in the KO mice. Data were analyzed using two-way ANOVA with Sidak’s multiple comparison corrections on the pre-planned contrasts examining the effect of TBI in the WT versus KO conditions. **p < 0.01, comparing KO (blue bars) to WT (orange bars). SSC side scatter

Back to article page