Fig. 2

HDAC3 inhibition reduces diabetes-induced BBB permeability and rescues junction protein expression in db/db mice. a db/db mice at the age of 16 weeks were treated with RGFP966 for 10 days. Fluorescent images of sodium fluorescein extravasation and CD31 staining. Images show × 4 macro-shots for the whole brain as well as zoomed in × 20 images in representative regions. b NaFl leakage was determined by quantifying the relative fluorescent intensity of sodium fluorescein. c Representative Western blot gel images of junction proteins ZO-1, VE-cadherin, Occludin, and Claudin-5. d–g Western blot analysis quantification for protein expression of ZO-1, VE-cadherin, Occludin, and Claudin-5. Data are expressed as mean ± SEM. *P < 0.05 vs. db/+, ^#P < 0.05 vs. db/db, n = 5 mice per group