Fig. 3

HDAC3 inhibition reduces hyperglycemia-IL1β (HG-IL1β) insult-induced permeability of cultured HBMEC monolayer. a Representative Western blot gel images of IL-1β expression in the mouse brain hippocampus and cortex tissues. b Quantification of Western blot analysis of IL-1β protein expression. Data are expressed as mean ± SEM. *P < 0.05 vs. db/+, n = 4; c. Relative HBMEC monolayer permeability after HG-IL1β insult with/without RGFP966 treatment. d TEER measurement of HBMEC monolayers after HG-IL1β insult with/without RGFP966 treatment. e Viability of cultured HBMEC after HG-IL1β insult with/without RGFP966 treatment. Data are expressed as mean ± SEM. *P < 0.05 vs. norm, ^#P < 0.05 vs. HG-IL1β, n = 6