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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Contribution of spinal cord glial cells to L. amazonensis experimental infection-induced pain in BALB/c mice

Fig. 3

Detection of spinal cord GFAP induced by i.pl. L. amazonensis infection. GFAP mRNA expression was determined in control non-infected and infected mice after the infection (5–40 days) by RT-qPCR (a). On day 30 after the infection (peak of GFAP mRNA expression), Western blot analysis of the whole spinal cord was conducted to confirm GFAP expression at day 30 post-infection (three mice per group, b and c). Subsequently, spinal cord samples were stained with primary antibody for astrocytes (GFAP, green) and regular nucleus (DAPI, blue) detection (four mice per group/four slides per mice). Representative immunostainings of the spinal cord of control non-infected and infected mice are shown in dk (d and e magnification × 10, scale bar 250 μm; panels fk magnification × 20, scale bar 100 μm, with gradual zoom for panels h and i and j and k). l The percentage of GFAP fluorescent intensity in each experimental group. Results are presented as mean ± SEM of six mice per group per experiment and are representative of two separated experiments for a. For b and c, results are presented as mean ± SEM of three mice per group and are representative of two separated experiments. For fl, results are presented as mean ± SEM of four mice per group and are representative of two separated experiments. ƒp < 0.05 compared to control non-infected, and 5, 10, and 20 days infected groups; *p < 0.05 compared to control non-infected mice (one-way ANOVA followed by Tukey post hoc)

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