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Fig. 4 | Journal of Neuroinflammation

Fig. 4

From: Contribution of spinal cord glial cells to L. amazonensis experimental infection-induced pain in BALB/c mice

Fig. 4

Detection of spinal cord Iba-1 induced by i.pl. L. amazonensis infection. Iba-1 mRNA expression was determined in control non-infected and infected mice after the infection (5–40 days) by RT-qPCR (a). On day 30 after the infection (peak of Iba-1 mRNA expression), Western blot analysis of the whole spinal cord was conducted to confirm Iba-1 expression at day 30 post-infection (four mice per group, b and c). Subsequently, spinal cord samples were stained with primary antibody for microglia (Iba-1, green) and regular nucleus (DAPI, blue) detection (four mice per group/four slides per mice). Representative immunostainings of the spinal cord of control non-infected and infected mice are shown in dk (d and e, magnification × 10, scale bar 250 μm; panels fk magnification × 20, scale bar 100 μm, with gradual zoom for panels h and i and j and k). l The percentage of Iba-1 fluorescent intensity in each experimental group. Results are presented as mean ± SEM of six mice per group per experiment and are representative of two separated experiments for a. For b and c, results are presented as mean ± SEM of four mice per group and are representative of two separated experiments. For panels fl, results are presented as mean ± SEM of four mice per group and are representative of two separated experiments. ƒp < 0.05 compared to control non-infected, and 5, 10 and 20 days infected groups; *p < 0.05 compared to control non-infected mice (one-way ANOVA followed by Tukey post hoc)

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