Fig. 9

L. amazonensis i.pl. infection decreases spinal cord total IκBα expression, and α-aminoadipate, minocycline, and PDTC i.t. treatments inhibit L. amazonensis-induced spinal cord NFκB activation. On day 30 after the infection, spinal cord total IκBα protein expression was evaluated by Western blot (four mice per group, a and b), and NFκB activation was evaluated in control non-infected and infected mice 7 h after treatments with vehicle, α-aminoadipate, minocycline, or PDTC by ELISA (c). Results are presented as mean ± SEM of four mice per group per experiment and are representative of two separate experiments for a and b. For c, results are presented as mean ± SEM of six mice per group and are representative of two separated experiments. *p < 0.05 compared to control non-infected mice; ^#p < 0.05 compared to vehicle treated-infected mice (one-way ANOVA followed by Tukey post hoc)