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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Meningitic Escherichia coli-induced upregulation of PDGF-B and ICAM-1 aggravates blood-brain barrier disruption and neuroinflammatory response

Fig. 3

PDGF-BB contributes to BBB disruption by downregulating TJ proteins. a Effects of the recombinant mouse PDGF-BB (10, 100, and 500 ng/mouse) on permeability of the mouse brain evaluated by Evan’s blue approach. b Mice were treated with or without PDGF-BB, and brains from both groups of mice were analyzed for the integrity of vascular endothelium by IF. The TJ proteins ZO-1, Occludin, and Claudin-5 were selected as the markers reflecting the integrity of the vascular endothelium. CD34 was specifically applied for labeling the microvessels. Scale bar indicates 50 μm. c Recombinant PDGF-BB treatment causes a significant and dose-dependent decrease of monolayer TEER based on ECIS system. Data were presented as mean ± SD from three independent assays. d Western blot analysis of ZO-1, Occludin, and Claudin-5 in hBMECs in response to multiple dosage of PDGF-BB (0, 5, 10, and 20 ng/mL). β-actin was used as the loading control, and densitometry was performed to analyze the differences. e Real-time PCR analysis of ZO-1, Occludin, and Claudin-5 transcription in hBMECs treated by multiple dosages of PDGF-BB. GAPDH was used as the internal reference. Data were presented as mean ± SD from three independent assays

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