Fig. 5

ICAM-1 expression was elevated in the brains of mice challenged by meningitic E. coli. a Real-time PCR determination of ICAM-1 transcription in brain total RNA from mice exhibiting different symptoms (n = 5). The transcription of β-actin was used as the internal reference. Data were presented as mean ± SD from five individual mice in each group. b Brain lysates from challenged mice with different signs were subjected to western blot analysis for expression of ICAM-1. β-actin expression was used as the loading control, and densitometry of the bands was performed to compare differences. c The brains of mild symptom and control mice were sectioned and analyzed for the expression of ICAM-1 via IF. The perivascular ICAM-1 was stained red. CD34 was specifically stained in green to label the blood vessel, and the cell nucleus was stained blue with DAPI. Scale bar indicates 50 μM. d The brains of mild symptom and control mice were subjected to ICAM-1 expression by IHC. ICAM-1 around the vessels was indicated by red arrows (infection group) or by black arrows (mock group). Scale bar indicates 50 μM