Fig. 2

Quantitative analysis of CXCL13, Chi3l4, and IgG1 expression in spinal cord samples from TMEV-IDD and R-EAE mice. Both RT-qPCR for mRNA and ELISA for protein of CXCL13 (a, d) and IgG1 (c, f) showed significant increases in expression in TMEV-IDD mice vs. both R-EAE and control mice. Conversely, both RT-qPCR for mRNA (b) and ELISA for protein (e) of Chi3l4 showed increased expression in R-EAE mice vs. both TMEV-IDD and control mice. RT-qPCR results are representative of 3 independent experiments (n = 31) for TMEV-IDD and 3 independent experiments (n = 16) for R-EAE. Likewise, ELISA results are representative of 3 independent experiments (n = 15) for TMEV-IDD and 2 independent experiments (n = 10) for R-EAE. Since the effect for age was not significant, sham mice were analyzed as all from one population (n = 14 for mRNA and n = 8 for proteins). Data are shown as mean ± SEM. ****p < 0.0001; *p < 0.05