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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Differential neuro-immune patterns in two clinically relevant murine models of multiple sclerosis

Fig. 3

B cell accumulation in spinal cords of R-EAE and TMEV-IDD. Total B cells and B cell phenotypes defined by IgD and IgM surface marker expression were identified by flow cytometry in spinal cords from TMEV-IDD mice (n = 22) at day 120 post-infection or R-EAE mice (n = 12) at day 14 post-immunization. a Representative plot showing minimal CD45hi infiltrating immune cells among total live cells in sham mice. b Representative gating strategy of CD45hi CD19+ B cells among total live cells isolated from TMEV-IDD or R-EAE spinal cords. Bar graphs depict mean percentage ± SEM (c) and mean number ± SEM (d) of CD45hi CD19+ B cells in TMEV-IDD or R-EAE spinal cords. e Representative smoothed plot discriminating IgD and IgM subsets including 1, IgD+IgM+ naïve/early activated; 2, IgDintIgM+ activated; 3, IgD-IgM+ transitional/ unswitched memory or ASC; and 4, IgD-IgM- switched memory/ASC B cells within CD45hi CD19+ cells in TMEV-IDD. f Bar graph depicting mean percentages ± SEM of B cell phenotypes discerned by IgD and IgM surface marker expression in TMEV-IDD and R-EAE. Statistical differences between TMEV-IDD and R-EAE spinal cords are indicated as determined by an unpaired t test. Data are representative of 3 independent experiments with 6 to 8 mice per experiments for TMEV-IDD and 2 independent experiments with 6 mice per experiment for R-EAE. ****p < 0.0001

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