Fig. 2

MC to neuronal [Ca²⁺]_i signaling. a Traces of [Ca²⁺]_i transients in a mast cell (red trace) and a hippocampal neuronal cell body (black trace) in response to mast cell activation by C48/80 microperfusion (100 μg/ml for 30 s). Inset shows pictures of MCs morphology and appearance before and after stimulation. b Traces of [Ca²⁺]_i transients in a mast cell (red trace) and a hippocampal neuron (black trace) in response to mast cell activation by FcεRI receptor crosslinking by DNP microperfusion (1 μg/ml for 30 s). c Traces of [Ca²⁺]_i transients in a MC (red trace) and a hippocampal neuronal cell body (black trace) in response to MC activation by LPS (1 μg/ml for 1 min). d Percentage of responding cells to C48/80, DNP, and LPS. e Mean [Ca²⁺]_i peak obtained in MCs (red bars) and hippocampal neurons (black bars) evoked by C48/80, DNP, and LPS. f Latencies measured by the time interval between the rise of MC [Ca²⁺]_i transient and the onset of neuronal [Ca²⁺]_i response