Fig. 5

PGs of CS and NMDA-receptors contribute to outline the [Ca²⁺]_i signal elicited by mast cells mediator cocktail. a Representative time course of the elevation of [Ca²⁺]_i upon application of a brief pulse of MCM to a neuronal soma. b The response was not changed after incubation with FSLLRY-NH2 and c Heparinase, but d was decreased when MCM was pre-digested with enzyme chondroitinase ABC. e, f Examples of [Ca²⁺]_i signals elicited after CNQX 20 μM and D-APV 50 μM treatment. g Averaged results on the magnitude of the peak of [Ca²⁺]_i and h area under curve are presented as percentage of means ± SEM. Chondroitinase inhibited partially the [Ca²⁺]_i signal as well as the NMDA receptor selective antagonist, D-APV, that blocked [Ca²⁺]_i response by 65%; however, the AMPA and kainate receptors blocker, CNQX, did not affect it. *p < 0.05; **p < 0.01