Skip to main content
Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: Reactive microglia and IL1β/IL-1R1-signaling mediate neuroprotection in excitotoxin-damaged mouse retina

Fig. 3

PLX5622-mediated ablation of microglia results in increased cell death and diminished neuronal survival in damaged retinas. Mice were fed control diet or diet that included PLX5622 for 2 weeks. After 2 weeks of treatment with PLX5622, eyes were injected with NMDA and retinas harvested at 1 (b, c) or 10 days (df) later. Retinal sections were labeled using the TUNEL method (b), antibodies to Iba1 (green; top) (a, d), Pax6 (green; middle) (d), or calretinin (red) (d).The box-plots c, e, and f illustrate the mean, upper extreme, lower extreme, upper quartile, and lower quartile (n ≥ 6 animals). Significance of difference (*p < 0.05) was determined by using a t test. The calibration bars panels a, b, and d represent 50 μm. Abbreviations: ONL, outer nuclear layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer

Back to article page